Rv0767c Transcriptional regulator, TetR family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0767c Transcriptional regulator, TetR family CDS 860069 860710 - 642 213 TRUE

Rv0767c (Transcriptional regulator, TetR family) is predicted to be co-regulated in modules bicluster_0152 with residual 0.50 and bicluster_0418 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.09 for bicluster_0152 and 0.00 and 7.60 for bicluster_0418 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 10 of 68
Gene Target Differential Expression Distance Expression pvalue Type
Transcriptional regulator, MarR family
Repressed -87 -0.65 0.00416848 CDS
Transcriptional regulator, MarR family
Repressed 36 -0.65 0.00416848 Internal.TSS
tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)
No 29 -0.34 0.134752 Internal.TSS
tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)
No 11 -0.34 0.134752 CDS
No -103 0.25 0.449302 Primary.TSS
Esterase LipW
Repressed -5 -2.91 7.61e-31 Primary.TSS
Possible membrane protein
No -98 -0.11 0.84006 CDS
No 70 -0.43 0.533536 Primary.TSS
Transmembrane transport protein MmpL1
No -11 -0.32 0.551448 CDS
Possible membrane protein
No -131 0.22 0.635672 CDS
Product (LegacyBRC) Product (RefSeq)
Uncharacterized HTH-type transcriptional regulator Rv0767c_MT0791
Operon # Operon
507 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607907 NP_215281.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426556 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426557 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426558 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0767c_B355 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.160000 0.80

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00000000000103042
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 9.09 U
D3I 3 3 3.83 9.40 I
D3U 3 3 3.83 8.83 U
D5I 9 5 6.00 7.76 I
D5U 17 5 6.00 10.15 U
D7I 18 7 8.14 5.92 I
D7U 19 7 8.14 9.03 U
D14I 4 14 15.63 1.95 I
D14U 4 14 15.63 7.19 U
D17I 3 17 19.15 I
D17U 3 17 19.15 7.17 U
D21I 4 21 23.23 1.11 I
D21U 4 21 23.23 6.00 U
D24I 3 24 26.60 I
D24U 3 24 26.60 6.70 U
D28I 4 28 30.61 1.86 I
D28U 4 28 30.61 5.86 U