Rv1332 Transcriptional regulatory protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1332 Transcriptional regulatory protein CDS 1500926 1501582 + 657 218 TRUE

Rv1332 (Transcriptional regulatory protein) is predicted to be co-regulated in modules bicluster_0136 with residual 0.42 and bicluster_0482 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0136 and 0.02 and 0.93 for bicluster_0482 respectively.

These modules are enriched for following go terms: cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, macromolecule metabolic process, cytoplasm.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-2.256 1500992 1500926
Displaying 1 - 5 of 5
Gene Target Differential Expression Distance Expression pvalue Type
No 67 -0.22 0.736174 CDS
Transcriptional repressor Mce3R, TetR family
No 41 0.1 0.874479 Primary.TSS
No results were found NA 16 Primary.TSS
Induced -124 0.93 0.0109368 CDS
PPE family protein
No 42 -0.84 0.336565 CDS
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, ArsR family
No -58 0.55 0.00000558 CDS
No -122 -0.09 0.688467 CDS
transcriptional regulator, ArsR family
No -132 0.18 0.127218 CDS
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv1332_MT1374 transcriptional regulatory protein
Operon # Operon
903 - - - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608472 NP_215848.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426655 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426656 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426657 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv1332_B437 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.510000 6.04

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00000000199904
p-value INH: 0.860973
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 14.97 U
D3I 3 3 3.83 15.14 I
D3U 3 3 3.83 14.69 U
D5I 9 5 6.00 15.40 I
D5U 17 5 6.00 15.04 U
D7I 18 7 8.14 15.46 I
D7U 19 7 8.14 14.89 U
D14I 4 14 15.63 15.85 I
D14U 4 14 15.63 14.60 U
D17I 3 17 19.15 15.69 I
D17U 3 17 19.15 14.05 U
D21I 4 21 23.23 15.67 I
D21U 4 21 23.23 14.09 U
D24I 3 24 26.60 15.26 I
D24U 3 24 26.60 13.34 U
D28I 4 28 30.61 15.35 I
D28U 4 28 30.61 13.22 U