Rv1338 Glutamate racemase (EC 5.1.1.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1338 murI Glutamate racemase (EC 5.1.1.3) CDS 1505075 1505890 + 816 271 FALSE

Rv1338 (Glutamate racemase (EC 5.1.1.3)) is predicted to be co-regulated in modules bicluster_0308 with residual 0.49 and bicluster_0386 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.32 for bicluster_0308 and 0.11 and 1,000.00 for bicluster_0386 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17858 MT1379 362
Product (LegacyBRC) Product (RefSeq)
Glutamate racemase glutamate racemase
Operon # Operon
903 - - - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Glutamate racemase D-Glutamine and D-glutamate metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

D-Glutamine and D-glutamate metabolism

3
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608478 NP_215854.1 Run
GO:0008881

glutamate racemase activity

glutamate racemase activity

Details: 
Catalysis of the reaction: L-glutamate = D-glutamate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008657

DNA topoisomerase (ATP-hydrolyzing) inhibitor activity

DNA topoisomerase (ATP-hydrolyzing) inhibitor activity

Details: 
Stops, prevents or reduces the activity of ATP-hydrolyzing DNA topoisomerase. ATP-hydrolyzing DNA topoisomerase catalyzes the DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined; product release is coupled to ATP binding and hydrolysis; changes the linking number in multiples of 2.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0008881

glutamate racemase activity

glutamate racemase activity

Details: 
Catalysis of the reaction: L-glutamate = D-glutamate.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: