Rv1339 Metal-dependent hydrolases of the beta-lactamase superfamily III

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1339 Metal-dependent hydrolases of the beta-lactamase superfamily III CDS 1505917 1506738 + 822 273 FALSE

Rv1339 (Metal-dependent hydrolases of the beta-lactamase superfamily III) is predicted to be co-regulated in modules bicluster_0030 with residual 0.60 and bicluster_0363 with residual 0.35.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.05 and 0.81 for bicluster_0030 and and for bicluster_0363 respectively.

These modules are enriched for following go terms: single-organism metabolic process, single-organism cellular process, biosynthetic process, cellular nitrogen compound metabolic pro..., nitrogen compound metabolic process, membrane part .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.594 51 1505968 36 1506004 1505917
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv1339_MT1380
Operon # Operon
903 - - - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608479 NP_215855.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: