Rv1334 Mec+

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1334 mec Mec+ CDS 1502641 1503081 + 441 146 FALSE

Rv1334 (Mec+) is predicted to be co-regulated in modules bicluster_0105 with residual 0.56 and bicluster_0308 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,200.00 and 5,800.00 for bicluster_0105 and 0.00 and 0.32 for bicluster_0308 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.887 1502668 1502641
Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14701 MT1376 110
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv1334_MT1376
Operon # Operon
903 - - - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608474 NP_215850.1 Run
GO:0008235

metalloexopeptidase activity

metalloexopeptidase activity

Details: 
Catalysis of the hydrolysis of a peptide bond not more than three residues from the N- or C-terminus of a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0008270

zinc ion binding

zinc ion binding

Details: 
Interacting selectively and non-covalently with zinc (Zn) ions.
GO Category: 
molecular_function
15
Total items in this category:  
GO:0019344

cysteine biosynthetic process

cysteine biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of cysteine, 2-amino-3-mercaptopropanoic acid.
GO Category: 
biological_process
4
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.140000 1.09

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: