Rv1380 Aspartate carbamoyltransferase (EC 2.1.3.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1380 pyrB Aspartate carbamoyltransferase (EC 2.1.3.2) CDS 1553232 1554191 + 960 319 FALSE

Rv1380 (Aspartate carbamoyltransferase (EC 2.1.3.2)) is predicted to be co-regulated in modules bicluster_0137 with residual 0.49 and bicluster_0339 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 850.00 and 1,200.00 for bicluster_0137 and 2,200.00 and 5,500.00 for bicluster_0339 respectively.

These modules are enriched for following go terms: 'de novo' pyrimidine nucleobase biosynth..., pyrimidine-containing compound biosynthe..., pyrimidine-containing compound metabolic..., organonitrogen compound metabolic proces..., nucleobase-containing small molecule met..., carbon-nitrogen ligase activity, with gl... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Aspartate carbamoyltransferase aspartate carbamoyltransferase catalytic subunit
Operon # Operon
928 - - - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608520 NP_215896.1 Run
GO:0004070

aspartate carbamoyltransferase activity

aspartate carbamoyltransferase activity

Details: 
Catalysis of the reaction: L-aspartate + carbamoyl phosphate = N-carbamoyl-L-aspartate + H(+) + phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: