Rv1384 Carbamoyl-phosphate synthase large chain (EC

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1384 carB Carbamoyl-phosphate synthase large chain (EC CDS 1557101 1560448 + 3 348 1 115 FALSE

Rv1384 (Carbamoyl-phosphate synthase large chain (EC is predicted to be co-regulated in modules bicluster_0104 with residual 0.59 and bicluster_0137 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,600.00 and 3,500.00 for bicluster_0104 and 850.00 and 1,200.00 for bicluster_0137 respectively.

These modules are enriched for following go terms: pyrimidine nucleobase biosynthetic proce..., pyrimidine nucleobase metabolic process, nucleobase biosynthetic process 'de novo' pyrimidine nucleobase biosynth..., pyrimidine-containing compound biosynthe..., pyrimidine-containing compound metabolic..., organonitrogen compound metabolic proces..., nucleobase-containing small molecule met..., carbon-nitrogen ligase activity, with gl....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Carbamoyl-phosphate synthase large chain carbamoyl phosphate synthase large subunit
Operon # Operon
928 - - - - - -
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Pyrimidine metabolism

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Alanine, aspartate and glutamate metabolism

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Metabolic pathways

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
57116856 YP_177804.1 Run

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

Catalysis of the reaction: 2 ATP + L-glutamine + CO2 + H2O = 2 ADP + phosphate + glutamate + carbamoyl phosphate.
GO Category: 
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plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
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No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: