Organism : Bacillus subtilis | Module List :
BSU25070 ispG

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq)

CircVis
Functional Annotations (8)
Function System
Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis cog/ cog
catalytic activity go/ molecular_function
terpenoid biosynthetic process go/ biological_process
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity go/ molecular_function
Terpenoid backbone biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
ispG_gcpE tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BSU25070
(Mouseover regulator name to see its description)

BSU25070 is regulated by 19 influences and regulates 0 modules.
Regulators for BSU25070 ispG (19)
Regulator Module Operator
BSU05170 200 tf
BSU05290 200 tf
BSU05970 200 tf
BSU08410 200 tf
BSU09380 200 tf
BSU18760 200 tf
BSU19540 200 tf
BSU25100 200 tf
BSU27000 200 tf
BSU40800 200 tf
BSU08520 151 tf
BSU13880 151 tf
BSU14990 151 tf
BSU19100 151 tf
BSU19200 151 tf
BSU23590 151 tf
BSU24520 151 tf
BSU25100 151 tf
BSU33740 151 tf

Warning: BSU25070 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
5252 7.70e-05 Tgagaat.aTTataA.ATaAg
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5253 9.00e+01 AGaAAggaA.G
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5344 1.70e-04 TttgaTaaAcgtgtTAtgATa
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5345 1.50e-06 gaAacagcAGCcttCCGCTttta
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BSU25070

BSU25070 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis cog/ cog
catalytic activity go/ molecular_function
terpenoid biosynthetic process go/ biological_process
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity go/ molecular_function
Terpenoid backbone biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
ispG_gcpE tigr/ tigrfam
Module neighborhood information for BSU25070

BSU25070 has total of 57 gene neighbors in modules 151, 200
Gene neighbors (57)
Gene Common Name Description Module membership
BSU00990 rpmG 50S ribosomal protein L33 (RefSeq) 64, 200
BSU02190 ybfF hypothetical protein (RefSeq) 178, 200
BSU02330 ybfQ hypothetical protein (RefSeq) 200, 389
BSU02690 ansZ L-asparaginase (putative lipoprotein) (RefSeq) 130, 200
BSU03970 ycnL putative reductase or disulfide isomerase (RefSeq) 151, 226
BSU04770 ydcH putative transcriptional regulator (RefSeq) 151, 258
BSU05120 cspC cold-shock protein (RefSeq) 151, 159
BSU05140 ydzE putative permease (RefSeq) 200, 389
BSU05290 ydeP putative transcriptional regulator (RefSeq) 151, 200
BSU05300 ydeQ putative NAD(P)H oxidoreductase (RefSeq) 151, 161
BSU07490 yfmF iron-dicitrate ABC transporter (ATP-binding protein) (RefSeq) 31, 151
BSU07500 yfmE iron-dicitrate ABC transporter (permease) (RefSeq) 66, 151
BSU07670 yflI hypothetical protein (RefSeq) 123, 151
BSU08410 yfiV putative transcriptional regulator (MarR family) (RefSeq) 200, 378
BSU08880 rpsN 30S ribosomal protein S14 (RefSeq) 123, 200
BSU09210 yhcT putative RNA pseudouridine synthase (RefSeq) 200, 301
BSU09220 yhcU hypothetical protein; orphan (RefSeq) 53, 200
BSU09810 yhaZ hypothetical protein (RefSeq) 178, 200
BSU13450 sigI putative RNA polymerase sigma factor SigI (RefSeq) 151, 379
BSU13810 ykvS hypothetical protein (RefSeq) 39, 200
BSU14080 ykuH hypothetical protein (RefSeq) 151, 157
BSU17270 ymaC putative phage-related replication protein (RefSeq) 200, 406
BSU17360 ymzA hypothetical protein (RefSeq) 95, 200
BSU17620 yncB DNA nuclease, lipoprotein (RefSeq) 151, 379
BSU17670 cotU spore coat protein (RefSeq) 44, 200
BSU18760 yoaU putative transcriptional regulator (LysR family) (RefSeq) 200, 239
BSU19540 yodB transcriptional repressor (RefSeq) 200, 389
BSU19550 yodC putative oxidoreductase (RefSeq) 200, 378
BSU22000 sspL small acid-soluble spore protein (RefSeq) 200, 239
BSU23000 ypbE hypothetical protein (RefSeq) 151, 311
BSU23010 ypbD putative membrane protease (RefSeq) 151, 226
BSU23020 recQ ATP-dependent DNA helicase (RefSeq) 151, 311
BSU23030 ypbB hypothetical protein (RefSeq) 151, 311
BSU23040 fer ferredoxin (RefSeq) 94, 151
BSU23590 ansR transcriptional regulator of ansAB (Xre family) (RefSeq) 151, 258
BSU25070 ispG 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq) 151, 200
BSU25090 yqfW putative nucleotidase (RefSeq) 151, 159
BSU25560 holA DNA polymerase III subunit delta (RefSeq) 151, 159
BSU27000 yraB putative transcriptional regulator (MerR family) (RefSeq) 123, 200
BSU28180 ysxD putative integral inner membrane protein (RefSeq) 151, 270
BSU28610 yshA cell division protein ZapA (RefSeq) 200, 226
BSU28620 rnhC ribonuclease HIII (RefSeq) 200, 311
BSU29920 ytmP putative kinase/phosphotransferase (RefSeq) 14, 151
BSU29970 ytkP putative cysteine synthase-like protein (RefSeq) 66, 151
BSU30070 opuD glycine betaine transporter (RefSeq) 14, 151
BSU33030 yuxN putative transcriptional regulator (RefSeq) 151, 216
BSU34490 yvdS hypothetical protein (RefSeq) 174, 200
BSU34500 yvdR hypothetical protein (RefSeq) 174, 200
BSU35530 tagO UDP-N-acetylglucosamine:undecaprenyl-P N-acetylglucosaminyl-1-P transferase (RefSeq) 151, 226
BSU35609 tuaA 200, 389
BSU37610 ywzC hypothetical protein (RefSeq) 151, 397
BSU38490 menA 1,4-dihydroxy-2-naphthoate octaprenyltransferase (RefSeq) 14, 151
BSU40720 yyaT putative acetyltransferase (RefSeq) 151, 270
BSU40730 yyaS putative integral inner membrane protein (RefSeq) 151, 270
BSU40740 yyaR putative acetyl-transferase (RefSeq) 200, 231
BSU40800 yyaN putative transcriptional regulator (MerR family) (RefSeq) 200, 238
BSU40810 yyaM putative efflux transporter (RefSeq) 200, 238
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BSU25070
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend