Organism : Bacillus subtilis | Module List :
BSU32500 pucB

enzyme for molybdopterin cofactor synthesis required for xanthine dehydrogenase (RefSeq)

CircVis
Functional Annotations (5)
Function System
Uncharacterized MobA-related protein cog/ cog
Purine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
pucB tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BSU32500
(Mouseover regulator name to see its description)

BSU32500 is regulated by 28 influences and regulates 0 modules.
Regulators for BSU32500 pucB (28)
Regulator Module Operator
BSU04680 29 tf
BSU04730 29 tf
BSU05700 29 tf
BSU09380 29 tf
BSU12510 29 tf
BSU26670 29 tf
BSU28400 29 tf
BSU28410 29 tf
BSU28820 29 tf
BSU31410 29 tf
BSU34200 29 tf
BSU36440 29 tf
BSU38220 29 tf
BSU40350 29 tf
BSU00800 231 tf
BSU02160 231 tf
BSU07010 231 tf
BSU09480 231 tf
BSU14740 231 tf
BSU18460 231 tf
BSU26220 231 tf
BSU26320 231 tf
BSU28410 231 tf
BSU32870 231 tf
BSU35490 231 tf
BSU37290 231 tf
BSU39850 231 tf
BSU40350 231 tf

Warning: BSU32500 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
5018 5.20e-12 aaaAAGGaGGA
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5019 2.00e+00 ag.gAaAaAatTgaAAAaAAC
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5406 1.10e+03 aCAGcCGaatCGcgCaG
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5407 1.10e+02 GcaAatatcgaaaGGagaAtG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BSU32500

BSU32500 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Uncharacterized MobA-related protein cog/ cog
Purine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
pucB tigr/ tigrfam
Module neighborhood information for BSU32500

BSU32500 has total of 52 gene neighbors in modules 29, 231
Gene neighbors (52)
Gene Common Name Description Module membership
BSU01590 ybaS putative sodium dependent transporter (RefSeq) 86, 231
BSU02110 ybyB hypothetical protein (RefSeq) 231, 299
BSU03130 nadE NAD synthetase (RefSeq) 29, 169
BSU03690 yczF hypothetical protein (RefSeq) 180, 231
BSU03900 gabT 4-aminobutyrate aminotransferase (RefSeq) 173, 231
BSU04410 ydbB putative enzyme ; cupin family (RefSeq) 29, 353
BSU04420 ydbC hypothetical protein (RefSeq) 29, 353
BSU05100 yddT hypothetical protein (RefSeq) 29, 299
BSU06170 ydjE putative sugar kinase (ribokinase family) (RefSeq) 222, 231
BSU10320 yhfP putative oxidoreductase (RefSeq) 29, 252
BSU12490 yjqC putative PBSX phage manganese-containing catalase (RefSeq) 231, 246
BSU13010 ykgB putative 6-phosphogluconolactonase (RefSeq) 29, 113
BSU13190 ispA intracellular serine protease (RefSeq) 222, 231
BSU14410 sipT type I signal peptidase (RefSeq) 165, 231
BSU14720 ylaB hypothetical protein (RefSeq) 89, 231
BSU14740 ylaD anti-YlaC sigma factor (RefSeq) 231, 361
BSU19800 phy phytase (RefSeq) 129, 231
BSU21320 yomL conserved hypothetical protein; phage SPbeta (RefSeq) 29, 299
BSU23080 aroD 3-dehydroquinate dehydratase (RefSeq) 128, 231
BSU24470 yqhS 3-dehydroquinate dehydratase (RefSeq) 29, 256
BSU25730 yqeC 6-phosphogluconate dehydrogenase-like protein (RefSeq) 231, 263
BSU27080 levR transcriptional regulator (NifA/NtrC family) (RefSeq) 219, 231
BSU27420 yrrI putative permease (RefSeq) 174, 231
BSU28340 ysnF putative stress response protein (RefSeq) 29, 299
BSU28410 gerE transcriptional regulator (RefSeq) 29, 248
BSU30250 ytaP putative hydrolase (RefSeq) 26, 231
BSU30650 dps DNA-protecting protein, ferritin (RefSeq) 29, 320
BSU32100 yumB putative NAD-disulfide oxidoreductase (RefSeq) 29, 391
BSU32230 yuxL putative acylaminoacyl-peptidase (RefSeq) 231, 285
BSU32410 pucH allantoinase (RefSeq) 231, 257
BSU32450 pucL urate oxidase with peroxide reductase N-terminal domain (RefSeq) 29, 231
BSU32460 pucM urate oxidase structural factor (RefSeq) 52, 231
BSU32500 pucB enzyme for molybdopterin cofactor synthesis required for xanthine dehydrogenase (RefSeq) 29, 231
BSU32510 pucA xanthine dehydrogenase molybdopterin recruitment factor (RefSeq) 86, 231
BSU33620 yvaK carboxylesterase (RefSeq) 29, 149
BSU34460 levB endolevanase (RefSeq) 130, 231
BSU34680 yvcT putative 2-hydroxyacid dehydrogenase (RefSeq) 29, 169
BSU35970 ywsB hypothetical protein (RefSeq) 29, 149
BSU37300 ywiC putative integral inner membrane protein (RefSeq) 29, 238
BSU37940 ywdJ putative purine/pyrimidine permease (RefSeq) 29, 243
BSU37950 ywdI hypothetical protein (RefSeq) 29, 243
BSU38190 galT galactose-1-phosphate uridylyltransferase (RefSeq) 29, 149
BSU38200 galK galactokinase (RefSeq) 29, 183
BSU38620 yxlJ 3-methyladenine DNA glycosylase (RefSeq) 29, 59
BSU38790 yxzE putative bacteriocin (RefSeq) 231, 406
BSU38970 yxjF 3-hydroxybutyrate dehydrogenase (RefSeq) 165, 231
BSU38980 scoB succinyl CoA:3-oxoacid CoA-transferase (subunit B) (RefSeq) 29, 231
BSU39560 yxeG putative integral inner membrane protein (RefSeq) 29, 233
BSU40430 yycE hypothetical protein (RefSeq) 156, 231
BSU40570 yybO putative permease (RefSeq) 130, 231
BSU40740 yyaR putative acetyl-transferase (RefSeq) 200, 231
VIMSS39609 VIMSS39609 None 2, 29
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BSU32500
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend