Organism : Bacillus subtilis | Module List :
BSU32470 pucE

xanthine dehydrogenase, iron-sulfur subunit (RefSeq)

CircVis
Functional Annotations (9)
Function System
Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs cog/ cog
electron transport go/ biological_process
electron carrier activity go/ molecular_function
metal ion binding go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
Purine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
pucE tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BSU32470
(Mouseover regulator name to see its description)

BSU32470 is regulated by 29 influences and regulates 0 modules.
Regulators for BSU32470 pucE (29)
Regulator Module Operator
BSU00370 326 tf
BSU02000 326 tf
BSU05370 326 tf
BSU06960 326 tf
BSU07010 326 tf
BSU07590 326 tf
BSU08100 326 tf
BSU09480 326 tf
BSU17590 326 tf
BSU23090 326 tf
BSU23100 326 tf
BSU26320 326 tf
BSU28410 326 tf
BSU39850 326 tf
BSU05370 52 tf
BSU07010 52 tf
BSU09500 52 tf
BSU10880 52 tf
BSU13870 52 tf
BSU25200 52 tf
BSU26220 52 tf
BSU26320 52 tf
BSU33660 52 tf
BSU33670 52 tf
BSU33970 52 tf
BSU36600 52 tf
BSU38450 52 tf
BSU38700 52 tf
BSU40800 52 tf

Warning: BSU32470 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
5062 2.00e-10 agAata.aagAaggaaagg.g
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5063 7.80e-01 tcA..atagag.a..gGctGt
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5590 3.80e-05 A.aAaaggGAGtTgaag
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5591 9.90e+01 AaGGAg
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BSU32470

BSU32470 is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs cog/ cog
electron transport go/ biological_process
electron carrier activity go/ molecular_function
metal ion binding go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
Purine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
pucE tigr/ tigrfam
Module neighborhood information for BSU32470

BSU32470 has total of 47 gene neighbors in modules 52, 326
Gene neighbors (47)
Gene Common Name Description Module membership
BSU02460 ycbC 5-dehydro-4-deoxyglucarate dehydratase (RefSeq) 168, 326
BSU02470 ycbD 2,5-dioxovalerate dehydrogenase (alpha-ketoglutaric semialdehyde dehydrogenase) (RefSeq) 168, 326
BSU02510 garD D-galactarate dehydratase (RefSeq) 180, 326
BSU05230 ydeK putative permease (RefSeq) 84, 326
BSU05490 ydfO putative dioxygenase (RefSeq) 52, 178
BSU05500 ydfP putative membrane bound oxidoreductase (RefSeq) 52, 178
BSU06150 gutB glucitol (sorbitol) dehydrogenase (RefSeq) 180, 326
BSU06160 gutP H+-glucitol symporter (RefSeq) 61, 326
BSU06300 cotA spore copper-dependent laccase (RefSeq) 84, 326
BSU10870 yisU putative aminoacid related metabolite efflux transporter (RefSeq) 52, 206
BSU10970 yitF putative enolase superfamily enzyme (RefSeq) 175, 326
BSU10980 yitG putative efflux transporter (RefSeq) 152, 326
BSU11900 yjcL putative integral inner membrane protein; possibly aquaporin-related (RefSeq) 52, 311
BSU11960 yjcR putative phage-related nucleic acid binding terminase small subunit (RefSeq) 52, 342
BSU13780 ykvP spore protein (RefSeq) 46, 52
BSU18180 yngB putative UTP-sugar-phosphate uridylyltransferase (RefSeq) 44, 52
BSU19510 yojB hypothetical protein (RefSeq) 21, 326
BSU19810 ypqP hypothetical protein (RefSeq) 86, 326
BSU20190 yosA hypothetical protein from phage SPbeta (RefSeq) 180, 326
BSU20710 yopZ conserved hypothetical protein; phage SPbeta (RefSeq) 44, 52
BSU24190 yqiI putative N-acetylmuramoyl-L-alanine amidase (RefSeq) 52, 285
BSU24200 yqiH putative lipoprotein (RefSeq) 44, 52
BSU26250 yqaN putative Holliday junction resolvase; skin element (RefSeq) 52, 206
BSU26360 yqaD conserved hypothetical protein; skin element (RefSeq) 52, 129
BSU32460 pucM urate oxidase structural factor (RefSeq) 52, 231
BSU32470 pucE xanthine dehydrogenase, iron-sulfur subunit (RefSeq) 52, 326
BSU32480 pucD xanthine dehydrogenase, substrate and molybdenum cofactor subunit (RefSeq) 52, 326
BSU32490 pucC xanthine dehydrogenase, FAD-binding subunit (RefSeq) 52, 326
BSU32890 yusQ putative tautomerase (RefSeq) 52, 389
BSU32900 yusR putative 3-oxoacyl-acyl-carrier protein reductase (RefSeq) 52, 389
BSU32910 yusS putative 3-oxoacyl-acyl-carrier protein reductase (RefSeq) 52, 389
BSU32920 yusT putative transcriptional regulator (LysR family) (RefSeq) 52, 389
BSU32970 yusY hypothetical protein (RefSeq) 31, 52
BSU32980 yusZ short chain dehydrogenase (RefSeq) 52, 285
BSU34080 yvfS putative ABC transporter (permease) (RefSeq) 318, 326
BSU34090 yvfR putative ABC efflux transporter (ATP-binding protein) (RefSeq) 318, 326
BSU34530 cotR spore coat protein assembly factor CotR (RefSeq) 281, 326
BSU34670 yvdA putative carbonic anhydrase (RefSeq) 26, 52
BSU34950 pelC secreted pectate lyase (RefSeq) 52, 61
BSU36030 ywrK putative Na+/H+ antiporter (RefSeq) 281, 326
BSU38660 yxlF putative ABC transporter component (ATP-binding protein) (RefSeq) 52, 164
BSU39350 hutH histidine ammonia-lyase (RefSeq) 85, 326
BSU39360 hutU urocanate hydratase (RefSeq) 85, 326
BSU39370 hutI imidazolonepropionase (RefSeq) 85, 326
BSU39380 hutG formimidoylglutamase (RefSeq) 85, 326
BSU39390 hutM histidine permease (RefSeq) 84, 326
BSU40560 yybP putative lipoprotein (RefSeq) 52, 178
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BSU32470
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend