This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 9.80 and 4,900.00 for bicluster_0220 and 0.00 and 0.00 for bicluster_0397 respectively.
These modules are enriched for following go terms: nitrogen compound metabolic process, carbon-oxygen lyase activity.
This gene is found to be non-essential for growth on cholesterol.
|Gene Target||Differential Expression||Distance||Expression||pvalue||Type|
|ChipSeq TF||Differential Expression||Distance||Expression||pvalue||Type|
|Motif 1||Motif 2||Residual|
|Product (LegacyBRC)||Product (RefSeq)|
|Uncharacterized protein Rv1990c_MT2044||transcriptional regulatory protein|
|BioCyc Gene Page||Cellular Overview Map|
|Cholesterol Essentiality||t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.